Fig. 3: Expression of the CD155 receptors TIGIT and CD96 on activated memory CD4+ T cells. | Nature Cardiovascular Research

Fig. 3: Expression of the CD155 receptors TIGIT and CD96 on activated memory CD4+ T cells.

From: Hyperactivity of the CD155 immune checkpoint suppresses anti-viral immunity in patients with coronary artery disease

Fig. 3

a–d, CD4+CD45RO+ memory T cells isolated from healthy individuals and patients with CAD were stimulated with anti-CD3/anti-CD28 for 5 days. Kinetics of TIGIT (a) and CD96 (b) transcript expression measured by RT–PCR (n = 4 patients and n = 4 controls). UMAP clustering of multi-parametric flow cytometry data. The expression of the co-inhibitory receptors TIGIT (c), CD96 (d), PD-1 (e) and CD226 (f) is indicated. Each dot plot concatenates data from six experiments. g,h, PBMCs were stimulated with SARS-CoV-2 proteins (1 μg ml−1) for 5 days. Total CD4+ T cells and antigen-reactive CD40L+ T cells were isolated, and TIGIT (g, n = 12) and CD96 (h, n = 16) transcripts were measured by RT–PCR. Data are mean ± s.e.m. Comparison by one-way ANOVA. P values are shown in each panel. CON, control; D, day.

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