Extended Data Fig. 8: Osmotic pump delivery of Rapamycin does not promote cardiomyocyte proliferation in adult heart.
From: Targeting calcineurin induces cardiomyocyte proliferation in adult mice
(a) Schematic of subcutaneously installed 14-day osmotic pump delivering Rapamycin (1.8 mg/kg/day) or DMSO vehicle control for 14 days from 9-11 weeks of age in CD-1 mice. (b) Immunostaining of hearts for cTnT (red) and pH3 (green), and quantification of mitotic cardiomyocytes (n = 4 for each group). The level of pH3+ cardiomyocytes observed in two of the rapamycin-treated sample was very low when compared to Fig. 3b. (c) Heart weight/Body weight of mice on DMSO (n = 5) and Rapamycin (n = 5). (b, c) Data are mean ± s.e.m.; unpaired two-sided t-test. Scale bar = 10μm.
