Extended Data Fig. 1: Ejection fraction and histology of CnB-iKO mice following gene deletion.
From: Targeting calcineurin induces cardiomyocyte proliferation in adult mice
Following gene deletion of CnB-iKO, hearts were assessed for: (a) Hoxb13 staining in control and CnB-iKO adult hearts at 2 weeks after gene deletion showing that Hoxb13 is not localized to myocardial nuclei in CnB-iKO hearts. Scale bars, 10 µm. (b) Ejection fraction was assessed up to 24 weeks after tamoxifen injections. (c) Heart weight at 2 weeks after gene deletion. (d) Body weight at 2 weeks after gene deletion. (e) Heart weight at 24 weeks after gene deletion; (f) Body weight at 24 weeks after gene deletion. (g) Heart weight/body weight at 24 weeks after gene deletion. (h) Hematoxylin and eosin stain (upper panels) and Masson trichrome staining (lower panels) at 2 weeks after gene deletion. Scale bars, 1 mm Data in a and h were independently repeated two times with similar results. Data are mean ± s.e.m.; unpaired two-sided t-test. *P < 0.05, **P < 0.01 (f). Sample numbers, n = 3 for each group (b, e, f, g), n = 12 for each group (c, d).
