Extended Data Fig. 2: Cardiomyocyte mitosis is increased after MI and CnB gene deletion.
From: Targeting calcineurin induces cardiomyocyte proliferation in adult mice
(a-b) At 1-week post MI, tamoxifen was administered to induce cardiomyocyte-specific deletion of CnB in CnB-iKO hearts and assessed at 4-weeks post MI: (a) schematic; (b) Immunostaining of hearts for cTnT (red) and pH3 (green), and quantification of mitotic cardiomyocytes for control and CnB-iKO hearts. (c-i) At 1-week post MI, tamoxifen was administered to induce cardiomyocyte-specific deletion of CnB in CnB-iKO hearts and assessed till 12-weeks post MI: (c) Heart weight; (d) Body weight; (e) Left ventricular end-diastolic anterior wall thickness (LVAW, d); (f) Left ventricular posterior wall end diastole (LVPW, d); (g) Left ventricular internal diameter end diastole (LVID, d); (h) Left ventricular internal diameter end systole (LVID, s); (i) Heart rate. Data are mean ± s.e.m.; unpaired two-sided t-test. *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 10 μm (b). Sample numbers, n = 7 for each group (c-i), n = 12 for each group (c, d), n = 3 for control and n = 5 for CnB-iKO (b).
