Extended Data Fig. 1: Rhythmic gene and protein level in mouse heart whole tissue extracts.
(a) WES analysis of REV-ERBα protein level in mouse hearts collected over a 24-hours period at rest phase (ZT0-ZT12) and active phase (ZT13-ZT24) (n = 4). HSP90α was used as a protein loading control. (b) WES analysis of REV-ERBα protein level in mouse hearts collected at ZT9 after vehicle or digoxin injection (0.1,0.5, and 0.1mpk at ZT5. (c) Cyclic Nr1d1, Nr1d2, Bmal1 and Cdkn1a mRNA expression in mouse hearts collected at rest (ZT0-ZT12) or active (ZT13-ZT24) phases Results are shown as mean + /−SEM (n = 3). (d) Nr1d1, Arntl (Bmal1) and Cdkn1a mRNA expression in mouse hearts collected at ZT9 after vehicle or digoxin (1mpk) injection at ZT5. Results are shown as mean + /−SEM (n = 8-9) which were compared using which were compared using a two-tailed Welch’s t-test. (e) P21 protein level in mouse hearts collected at ZT9 after vehicle or digoxin (1mpk) injection at ZT5. (f) REV-ERBα protein level in mouse hearts collected at ZT0 after vehicle or digoxin (1mpk) injection at ZT20. Basal REV-ERBα protein level at ZT9 is shown here as reference. Measurements were from distinct samples. Quantification of data in panels (a), (b) and (c) appears in Fig. 1.
