Fig. 1: Demonstration of live-cell PALM imaging in RFP RyR cardiomyocytes.
a, Effects of photoactivation. Minimal fluorescence was observed at 543 nm at baseline (left). Photoactivation was achieved using brief (30 s) low-intensity illumination with a 405 nm confocal laser, resulting in a robust fluorescence signal increase within the illuminated band across the center of the cell. An intensity plot measured along the dotted orange line illustrates regional RyR photoactivation (right). b, Super-resolution RyR images obtained by fixed-cell surface dSTORM (left) and live-cell TIRF–PALM (middle). dSTORM and PALM measurements showed similar RyR organization on the cell surface, as indicated by measurements of cluster sizes (right). Data are presented as mean ± s.e.m. Difference between groups was tested with two-tailed linear mixed models nested by cell and animal levels. ndSTORM = 3 animals, 20 cells; nPALM = 3 animals, 20 cells.
