Extended Data Fig. 2: Validation of normal animal growth and cardiomyocyte Ca2+ homeostasis in transgenic animals. | Nature Cardiovascular Research

Extended Data Fig. 2: Validation of normal animal growth and cardiomyocyte Ca2+ homeostasis in transgenic animals.

From: Live-cell photoactivated localization microscopy correlates nanoscale ryanodine receptor configuration to calcium sparks in cardiomyocytes

Extended Data Fig. 2

(a) Animal body and heart weights were similar in knock-in (TG) and wild-type (WT) animals. n = 29 animals; 5 male WT, 6 female WT, 7 male TG, 10 female TG. (b) Ca2+ transients were measured in isolated cardiomyocytes using confocal line-scan imaging during 1 Hz field stimulation. Representative line-scans are show in the upper panels, with spatially-averaged Ca2+ transients below. (c) Mean measurements of Ca2+ transient parameters were similar in TG and WT cardiomyocytes. RT50 = time to half decline. TTP = time to peak. nrecordings: control = 42, TG = 39.

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