Extended Data Fig. 3: RyR cluster size measurements and resolution obtained in dSTORM and PALM imaging experiments.
(a) Distribution of RyR cluster size measurements from dSTORM imaging at the cell surface. (b) Control experiments performed with secondary antibody alone indicated nonspecific labeling, artefactually manifested as small RyR clusters. (c) Distribution of RyR cluster size measurements by PALM imaging at the cell surface. In comparison with dSTORM results, proportionally fewer small RyR clusters were identified by PALM imaging, as issues with non-specificity of antibody labeling were avoided. (d) Resolution obtained for dSTORM and PALM imaging, as estimated by Fourier ring correlation47. Data are mean ± standard error. n = 10 PALM images, 7 dSTORM images.
