Extended Data Fig. 6: Sensitivity analysis of the spark fitting algorithm. | Nature Cardiovascular Research

Extended Data Fig. 6: Sensitivity analysis of the spark fitting algorithm.

From: Live-cell photoactivated localization microscopy correlates nanoscale ryanodine receptor configuration to calcium sparks in cardiomyocytes

Extended Data Fig. 6

(a) Spark fitting performance is illustrated for two synthetic example sparks (left panels). Sparks were degraded by Poisson and Gaussian noise, blurred, and then fitted by a 2D elliptical Gaussian function as described in Methods. Black and red contours (from outside in) in the right panel correspond to 10%, 34.1% (one SD), 50%, and 75% of the amplitude of the ground truth and fitted result, respectively. (b) Fitting error (δ) indicating deviation from ground truth, based on 2000 simulations at fixed F0 and spark amplitude (40 and 1.0, respectively). Measurements are presented for (from left to right), angle of rotation (θ), x and y positions of the centroid (x0 and y0), spark amplitude, and spark width in x and y dimensions (σx and σy). (c), (d) show changes in average deviance (squares) and SD (shaded) of the fitting error over a range of F0 and amplitudes, respectively. Each data point is derived from 2000 simulations.

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