Extended Data Fig. 1: Knock-in strategy and validation.

(a) Schematic detailing the insertion plan using homologous recombination and neomycin cassette (provided by Cyagen, Santa Clara, CA, USA). (b) PCR analyses were employed to genotype mouse progeny, based on the presence of the WT (0) RyR allele (281 base pairs) or PA-Tag RFP knock-in transgene (TG, 413 base pairs). Randomly-selected mice examined in this example blot include those expressing the homozygous TG, which were used in experiments (animals #26 and 27), a heterozygous animal (#28), and an animal null for the TG (#29). TG gene expression was validated in all experimental animals.