Extended Data Fig. 8: Effect of Tie2 silencing on gene expression in cultured Tie2 + FBs.
From: The angiopoietin receptor Tie2 is atheroprotective in arterial endothelium
a. Quantifications of Tie2 (Tek) mRNA level in cultured Tie2+ FBs and Tie2- FBs (Passage 6) in comparison with murine endothelial cell line Bend.3 (n = 3 independent mice per group). b. Quantification of Tie2 (Tek) mRNA level in the Tie2+ FBs transduced with shTie2-A or shTie2-D vs scramble (SCR) control (n = 3 independent mice per group). c. Heatmap showing upregulated transcripts related to inflammatory cell migration, immune response, inflammatory response, and chemokine and chemotaxis pathways in the Tie2-silenced Tie2+FBs. d. Violin plots showing the upregulation of Ccl7, Ccl8, Pycard, C3, Socs1, Apod, and Il6 in the Tie2+ fibroblasts during feeding Western diet in Tie2WT mice. e. Circle plot showing the significant communications in the IL6 signaling pathway and CCL signaling pathway analyzed using CellChat. f. Quantification of CCL5 secreted in the Tie2+ fibroblasts after Tie2 silencing (n = 3 independent samples in SCR, n = 2 independent samples in shTie2-A, n = 3 independent samples in shTie2-D; 6 h: P = 0.0016, 12 h: P = 3.59 × 10−9, 18 h: P = 3.0 × 10−14, 12 h: P = 2.9 × 10−14). Values show mean ± s.d. Statistical significance was determined using Two-way ANOVA with Geisser-Greenhouse correction and Turkey’s multiple comparisons test to the scramble control.
