Fig. 5: Tie2 restricts inflammatory signaling in the Tie2+ fibroblasts.
From: The angiopoietin receptor Tie2 is atheroprotective in arterial endothelium
a, UMAP plots of aortic cells from adult Tie2WT mice that received normal diet and Tie2WT mice after 20 weeks of Western diet. b, Dot plots showing top changed genes in the top regulated pathways in the Tie2+ fibroblasts. c, Heatmap showing upregulated genes related to inflammatory cell migration, immune response, inflammatory response, and chemokine and chemotaxis pathways upon Tie2 silencing. d, Gene ontology analysis on the GO terms that were upregulated in the Tie2-silenced Tie2+ fibroblasts. Bonferroni correction (multiple comparison test) was applied, followed by Benjamin–Hochberg adjusted P value or FDR values to plot the gene ontology. e, Quantification of IL-6 secreted in the Tie2+ fibroblasts after Tie2 silencing (n = 3 independent samples in SCR, n = 2 independent samples in shTie2-A and n = 3 independent samples in shTie2-D; 3 h, P = 0.0281, 6 h, P = 0.000102; 12 h, P = 3.21 × 10−8; 18 h, P = 2.16 × 10−13; 24 h, P = 3.5 × 10−14). Values show mean ± s.d. Statistical significance was determined using two-way ANOVA with Greenhouse–Geisser correction and Tukey’s multiple comparisons test.
