Fig. 6: Tie2 function dominates over Tie1 function in atherosclerosis development.

a, Representative images of Tie1^WTTie2^WT and Tie1;Tie2^iΔAEC (n = 7 independent Tie1^WTTie2^WT mice and n = 10 independent Tie1;Tie2^iΔAEC mice) aortas stained with Sudan IV (scale bar, 2 mm). b, Quantification of lesion areas in the Tie1^WTTie2^WT and Tie1;Tie2^iΔAEC aortas (n = 7 independent Tie1^WTTie2^WT mice and n = 10 independent Tie1;Tie2^iΔAEC mice over two independent experiments; total, P = 0.0132; thoracic aorta, P = 0.0012; abdominal aorta, P = 0.0063). Values show mean ± s.d. Statistical significance was determined using Student’s t-test (two-tailed, unpaired). c, Quantification of CD68⁺ area (n = 5 independent Tie1^WTTie2^WT mice and n = 4 independent Tie1;Tie2^iΔAEC mice; P = 0.0081). Values show mean ± s.d. Statistical significance was determined using Student’s t-test (two-tailed, unpaired). d, Representative immunofluorescence images of the Tie1^WTTie2^WT and Tie1;Tie2^iΔAEC aorta sections stained for PECAM1 (green), DAPI (blue) and VCAM1 (red). e, Quantifications of VCAM1 in the Tie1^WTTie2^WT and Tie1;Tie2^iΔAEC aortas (n = 5 independent Tie1^WTTie2^WT mice and n = 4 independent Tie1;Tie2^iΔAEC mice). Values show mean ± s.d. Statistical significance was determined using Student’s t-test (two-tailed, unpaired). Scale bar, 100 μm.

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