Extended Data Fig. 7: Immunolocalization reveals unique vascular connections in the mouse nasal mucosa.
From: Three-dimensional morphologic and molecular atlases of nasal vasculature
a, Images showing immunolocalization of nasal vasculature by simultaneous i.v. injection of fluorescence-labeled anti-podocalyxin (PODXL) (for detection of arterioles and capillary), anti-PLVAP (for detection of capillary and vein), and anti-CD31 (all vessels) antibodies to Prox1-GFP reporter mice. Scale bars, 100 μm. b, Four different single-channel images from the merged channel image of a are shown. Note that venule (yellow arrowheads and yellow lines; Prox1-/PODXL−/PLVAP+/CD31+) is located between capillaries (Prox1−/PODXL+/PLVAP+/CD31+) and VSs (white arrowheads and white lines; Prox1+/ PODXL−/PLVAP−/CD31+). Scale bars, 100 μm. Two independent experiments in n = 3 mice show similar findings.
