Fig. 2: Distributions of typical and atypical LVs in the mouse nasal cavity.

a, Image showing distributions of Prox1⁺/LYVE1⁺/VEGFR3⁺ LVs, Prox1⁺/LYVE1⁻/VEGFR3⁺ LVs and Prox1⁺ VSs at the indicated areas and regions of whole-mounted lateral side of nasal cavity of Prox1-GFP reporter mice. Scale bars, 500 μm. b–e, Images showing distributions of Prox1⁺ VSs at the middle portion and Prox1⁺/LYVE1⁻/VEGFR3⁺ LVs at the base portion of the meatus in the nasal mucosa (b), Prox1⁺/LYVE1⁺/VEGFR3⁺ LVs at anterior portion and around NALT (c) and LYVE1⁺/CCL21⁺ LVs and LYVE1⁻/CCL21⁺ LVs in the respiratory mucosa (d,e). Note the lymphatic capillary having sharp blunt-ends (light yellow arrowheads), lymphatic capillary having a round blunt-end (violet arrowheads) and LYVE1⁺ collecting LVs (blue arrowheads). VE-cad, VE-cadherin. Scale bars, 100 μm. f, Images showing button-like intercellular junctions (upper panels) in Prox1⁺/LYVE1⁺ LVs and zipper-like intercellular junctions (lower panels) in Prox1⁺/VEGFR3⁺ LVs. Scale bars, 50 μm. a–f, Similar findings were obtained from n = 6–8 mice per group from 3–5 independent experiments. g, Diagrams depicting two types of lymphatic capillary ends and distributions of two types of LVs in the nasal cavity. h, Comparison of the area between type 1 (LYVE1⁻/VEGFR3⁺) LVs and type 2 (LYVE1⁺/VEGFR3⁺) LVs in the nasal mucosa. Each dot indicates a value from one mouse and n = 4 mice from two independent experiments. Bars indicate mean ± s.d. P values versus type1 LVs by two-tailed Mann–Whitney U-test.

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