Fig. 5: PROX1+ VSs and LVs in the human nasal mucosa.
From: Three-dimensional morphologic and molecular atlases of nasal vasculature
a,b, Diagram showing the tissue sampling of human nasal mucosa and images and quantification of FOXC2+/CD31+ VS (blue arrowheads) and PDPN+/CD31+ LVs (green arrowheads). The anti-FOXC2 antibody produces a non-specific positive signal in the mucosal glands (red arrowheads), presumably because of the mucus’s stickiness. Scale bar, 500 μm. Each dot in b indicates a value from each patient and n = 4 patients. Bars indicate mean ± s.d. P values versus VS by two-tailed Mann–Whitney U-test. c, UMAP plot visualizing heterogeneity of EC subpopulations in the human nasal mucosa. d, UMAP plot depicting normalized expression levels of GJA4, PROX1, FOXC2, VCAM1, NR2F2 and VWF in the indicated clusters. e, Diagram and UMAP plot showing the reference mapping of human nasal ECs using mouse nasal ECs as a reference. f, Heat map showing degrees of correlations between the annotations and the reference mapping in e. g, UMAP plot visualizing similar EC heterogeneity between male and female patients (each n = 2). h, Violin plots showing exclusive expression of indicated genes in lymphatic EC cluster.
