Fig. 6: OVA-AR mouse model shows VS contraction and increased lymphoid cell infiltrations in the nasal mucosa.
From: Three-dimensional morphologic and molecular atlases of nasal vasculature
a, Diagram depicting the generation of OVA-AR model in adult Prox1−GFP mice by i.p. administrations of OVA (1-week interval) followed by i.n. administrations of PBS or OVA (1-day interval). i.p. administration of PBS or the cocktail blocking antibodies against LFA1 and VLA4 (L/V) was performed from the starting day of i.n. administration of OVA. b, Images showing distributions of Prox1+ VSs, endomucin+ capillaries and CD3e+ T cells of whole-mounted lateral side nasal cavity of Prox1-GFP reporter mice. M-T, maxillo-turbinate. Note reduced area and diameter of Prox1+ VSs in the OVA-AR group. Scale bars, 500 μm. Three independent experiments in n = 4 mice showed similar findings. c, Images and comparison of VCAM1 in the Prox1+ VSs. Scale bars, 100 μm. VCAM1 intensity was normalized on the control intensity values. Each dot indicates a value from one mouse and n = 4 mice per group from three independent experiments. Bars indicate mean ± s.d. P values versus control by two-tailed Mann–Whitney U-test. d, Images and comparisons of distributions of CD3e+ T cells and B220+ B cells and area and diameter of Prox1+ VSs. Scale bars, 100 μm. Each dot indicates a value from one mouse and n = 4–5 mice per group from three independent experiments. Bars indicate mean ± s.d. P values versus control by two-tailed Mann–Whitney U-test. NS, not significant.
