Fig. 7: Prox1+ VS serves as a major vessel for leukocyte trafficking in the nasal vasculature, and OVA-AR induces regression of type 1 LVs but increases immune cell infiltration within the LVs.
From: Three-dimensional morphologic and molecular atlases of nasal vasculature
a, Diagram depicting the generation of OVA-AR model in adult Prox1-GFP by i.p. and i.n. administrations of OVA. i.p. administration of blocking antibody against VLA4 was performed. In total, 1 × 107 of LeukocytesRed was administered through the tail vein 1 hour after the i.n. OVA administration. b,c, Images and comparison of the number of vascular-adherent LeukocytesRed between Prox1+ VS and endomucin+ capillary. Scale bars, 50 μm. Each dot indicates a value from one mouse and n = 4 mice from two independent experiments. Bars indicate mean ± s.d. P values versus Prox1+ VS by two-tailed Mann–Whitney U-test. d, 3D image showing localization of vascular-adherent LeukocytesRed within Prox1+ VS. Scale bar, 50 μm. e,f, Images and comparison of the number of vascular-adherent LeukocytesRed in Prox1+ VS between OVA-AR and OVR-AR + VLA4. Scale bars, 100 μm. Each dot indicates a value from one mouse and n = 4 mice per group from two independent experiments. Bars indicate mean ± s.d. P values versus OVA-AR by two-tailed Mann–Whitney U-test. g,h, Images and comparison of the density of LYVE1−/VEGFR3+ type1 LVs (yellow arrowheads) in the nasal mucosa between control and OVA-AR mice. Scale bars, 200 μm. Each dot indicates a value from one mouse and n = 4 mice per group from two independent experiments. Bars indicate mean ± s.d. P values versus control by two-tailed Mann–Whitney U-test. i, Representative images showing no change of the zipper-like EC junction in type 1 LVs between control and OVA-AR mice. Scale bars, 50 μm. Two independent experiments in n = 4 mice showed similar findings. j,k, Images and comparisons of CD3e+ T cells (dark-red arrowheads) and MHCII+ cells (blue arrowheads) within type1 LVs in the nasal mucosa between control and OVA-AR mice. Scale bars, 100 μm. Each dot indicates a value from one mouse and n = 4 mice per group from two independent experiments. Bars are mean ± s.d. P values versus control by two-tailed Mann–Whitney U-test.
