Extended Data Fig. 2
From: Blockade of IL-6 signaling alleviates atherosclerosis in Tet2-deficient clonal hematopoiesis
a. Gating strategy for LSK cells and Hematopoietic progenitor cells (HPC, the first row), LSK (Sca1+c-Kit+) subpopulation (middle row) and HPC (Sca1−c-Kit+) subpopulation (the third row). For the LSK subpopulation, CD150+CD48−, CD150−CD48−, CD150−CD48+ LSK fractions are long term HSC (LT-HSC), short term HSC (ST-HSC) and multipotent progenitors (MPP) respectively. For the HPC subpopulation, the cells are defined as common myeloid progenitors (Lin−Sca1−c-Kit+CD34+CD16/32int; CMP), granulocyte and macrophage progenitors (Lin−Sca1−c-Kit+CD34+CD16/32hi, GMP). b. Hematopoietic stem and progenitor cells in BM. n = 12 mice per group. One-way ANOVA followed by Tukey’s multiple-comparison test. c-h. CSF1R expression on stem and progenitor cells as determined by flow cytometry shown as mean fluorescence intensity and percentage of positive cells. n = 12 mice per group. One-way ANOVA followed by Tukey’s multiple-comparison test. All the Data are Mean ± SEM.
