Fig. 3: Absolute lipid quantification during megakaryopoiesis reveals a stable lipidome and distinct clusters of regulation.
From: Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
a, Venn diagram of shared and distinct lipids across the time course of differentiation. b, Bar graph of significant regulated and nonregulated lipids during megakaryopoiesis. c, Quantitative proportion of regulated lipids on the overall observed concentration changes. d, Analysis of the fraction of correlations connecting lipids of the same lipid class (red) or different lipid classes (blue), as a function of correlation strength. e, Hierarchical clustering of the lipid–lipid correlation and anticorrelation matrix. Rows and columns correspond to the 506 quantified lipid species. Black boxes indicate clusters of strongly correlated and anticorrelated lipids with a Pearson correlation coefficient (r) of 0.99 or above. Lipid cluster numbers are indicated on the right. Number of lipids in each cluster are sorted by lipid class. f, Network visualization of the lipid–lipid correlations. Nodes are individual lipid species. Edges are correlations or anticorrelations (r ≥ 0.99). Node size represents the degree of connectivity, and node color represents the analyzed lipid class. g,h, The network during differentiation. Fold change was mapped onto the generated network and day 1 compared to day 3 (g) and day 3 to day 7 (h). The displayed pure red indicates a fold change of ≥2, and pure blue indicates a fold change of ≤−2. Data are combined from three independent biological experiments, and mean values are shown.
