Fig. 7: Lipid-driven functional regulation and underlying mechanisms of MK maturation and thrombopoiesis.
From: Critical shifts in lipid metabolism promote megakaryocyte differentiation and proplatelet formation
This study focuses on the functional analysis and regulation of MK maturation and proplatelet formation, using a multiomics approach and incorporating both in vitro and in vivo methodologies. To develop the multiomics method, we isolated hematopoietic stem cells from murine bone marrow and subjected them to a 7-day differentiation protocol with TPO. The SIMPLEX workflow, which enables simultaneous lipid and protein sample preparation, was used to comprehensively determine the general molecular composition of MKs. The results revealed significant anionic lipid membrane remodeling and relocalization of the CKIP-1/CK2α complex to the plasma membrane, which appear to be essential for adequate platelet biogenesis. The graphical illustration was generated using BioRender.
