Extended Data Fig. 1: Stirred tank bioreactor production of PSC-CMs for porcine transplantation experiments.

(a) Schematic depicting PSC-CM stirred tank reactor production protocol. (b) Appearance of cells at day 0 and (c) day 15 of differentiation protocol. (d) Immunostaining for cardiac troponin T demonstrating evidence of cytoskeletal formation in PSC-CMs. (e) Representative flow cytometric evaluation of PSC-CMs quantifying cardiac troponin T expression. (f) qPCR data showing expression profile of pluripotency, (g) mesodermal, (h) cardiac progenitor, and (i) cardiomyocyte markers over time course of differentiation protocol. All data in panels (f-i) is presented as mean ± SEM. n = 5 biologically independent experiments for each marker.