Extended Data Table 3 Antibodies used in this study

From: Bone morphogenic protein-4 availability in the cardiac microenvironment controls inflammation and fibrosis in autoimmune myocarditis

Clone

Reagent

Conjugate

Concentration (µg ml−1)

Source

IA8

anti-Ly6G

PE

1

Pharmingen

AL-21

anti-Ly6C

PerCP Cy5

2

BD Biosciences

145-2C11

anti-CD3e

PerCP

5

BioLegend

30-F11

anti-CD45

APC-Cy7

2

BioLegend

30-F11

anti-CD45

PE

2

BioLegend

B20.1

anti-Vα2 TCR

APC

2

BD Biosciences

MR5-2

anti-Vβ8.1/8.2 TCR

FITC

5

BD Biosciences

RM4-5

anti-CD4

BV-605

2

BioLegend

XMG1.2

anti-IFNγ

APC

2

BioLegend

TC11-18H10.1

anti-IL17

PE

2

BioLegend

2G9

anti-IA/IE

FITC

2

BD Biosciences

SA011F11

anti-CX3CR1

APC-Fire750

2

BioLegend

SA203G11

anti-CCR2

BV-421

2

BioLegend

X54-5/7.1

anti-CD64

PeCy7

0.8

BioLegend

MEC13.3

anti-CD31

A647

2

BioLegend

MEC14.7

anti-CD34

BV421

2

BioLegend

104

anti-CD45.2

BV510

2

BioLegend

AMS-32.1

anti-IAd

PE

1

BD Biosciences

3E2

anti-ICAM-1/CD54

BV421

2

BD Biosciences

APA5

anti-PDGFRα/CD140α

BV605

2

BD Biosciences

BP3

CD157/BST1

PE

2

BioLegend

8.1.1

anti-PDPN

PeCy7

2

BioLegend

563260

streptavidin

BV605

2

BD Biosciences

D7

anti-Sca-1/Ly6A/E

PerCP

2

BioLegend

RM-45

anti-CD4

Alexa 488

1

BioLegend

RAM-34

anti-CD34

Biotin

1

Invitrogen

M1/70

anti-CD11b

Alexa 647

1

BioLegend

30-F11

anti-CD45

Alexa 488

 

BioLegend

Ab155033

rabbit anti-BMP4

None

1

Abcam

AB-758

goat anti-COL1

None

0.8

Merck

711-165-152

donkey anti-rabbit

Cy3

1

Jackson ImmunoResearch

705-605-147

donkey anti-goat

Alexa 647

1

Jackson ImmunoResearch

016-600-984

streptavidin

Alexa 647

1

Jackson ImmunoResearch

TER-119

anti-Ter119

BV510

2

BioLegend

MVCAM.A

anti-VCAM-1/CD106

Biotin

2

BioLegend

Monoclonal antibody 13820

anti-pSMAD1/5/9

PE

1

Cell Signaling Technology

  1. Antibodies used in this study for confocal microscopy and flow cytometry.