Fig. 5: zTPM standardization predicts that SRC but not BLK is actively expressed in ECs.
Number of datasets analyzed across EC subtypes for each of the five genes, n = 220. a, Stacked bar charts depicting the total number of datasets per EC subtype in which each indicated gene was detected (>−2.38 zTPM) or not detected (≤−2.38 zTPM) above the previously determined active expression threshold of −2.38 zTPM for HUVECs. The percentage of datasets in which the indicated gene was expressed above the threshold is reported below each bar for the corresponding EC subtype. HUVEC n = 127, HDMEC n = 14, mouse lung EC n = 15, mouse brain EC n = 46, mouse retina EC n = 18. b, zTPM values for the indicated genes in each dataset with expression >0 TPM, split by EC subtype. The TPM values for each dataset are available in the corresponding source data file. Each data point represents one dataset; values are shown together with boxplots to illustrate the median (center line) and interquartile range (box limits) (for n, see the corresponding source data file). The red dashed line indicates the −2.38 zTPM threshold above which a gene is considered actively expressed in HUVECs. Lung, brain and retina EC data are from mouse datasets.
