Extended Data Fig. 5: Neutrophil-released NETs after tissue injury contribute to reduced B and T cell numbers in PP.

a, A sample-wise comparison showed a Pearson correlation coefficient for all analyzed samples for mass spectrometry analysis. b, Relative plasma levels of citH3−DNA complexes after 6 h of myocardial infarction or sham operation (n = 5 per group, two−tailed Mann−Whitney U test, **P = 0.0079). c, Relative frequencies of blood Ly6G⁺CD11b⁺ neutrophils in stroke mice treated with anti−Ly6G antibodies or vehicle (n = 5−7 per group, two−tailed Mann−Whitney U test, **P = 0.0025). d, Quantification of plasma DNA at 6 h and 24 h after stroke + isotype antibody or stroke + anti−Ly6G antibody injected mice (n = 5−14 per group, ordinary one−way ANOVA, ****P < 0.0001, Ly6G antibody 6 h vs 24 h P > 0.9999). e, The total numbers of CD3⁺ T cells in PP of stroke + isotype antibody or stroke + anti−Ly6G antibody−injected mice after 24 h (n = 5−7 per group, two−tailed Mann−Whitney U test, **P = 0.0025). f, Brain infarct volumes after 24 h of stroke + isotype antibody or stroke + anti−Ly6G antibody−injected mice (n = 5−7 per group, two−tailed Mann−Whitney U test, P = 0.3434). g, 3D reconstruction images of CD19⁺ B cells and CD3⁺ T cells in PP isolated from duodenum, jejunum and ileum 24 h of stroke + isotype antibody or stroke + anti−Ly6G treated mice, scale bar=500 μm. Data represented as mean ±s.d., Shapiro-Wilk normality. PP=Peyer’s patch, CitH3=citrullinated Histone-3.