Extended Data Fig. 9: Inhibition of NETs release after stroke prevented lymphocyte loss in PP and spleen in young and old mice.

a, Numbers of CD19⁺ B cells and CD3⁺ T cells in PP of sham, stroke and stroke + LDC7559 treated young mice (n = 5−6 mice per group, two−tailed Mann−Whitney U test, CD19⁺ **P = 0.0043, CD3⁺ **P = 0.0022). b, Numbers of CD19⁺ B cells and CD3⁺ T cells in spleens of sham, stroke and stroke + LDC7559 treated young mice (n = 6−7 mice per group, two−tailed Mann−Whitney U test, **P = 0.0012). c, Numbers of CD19⁺ B cells and CD3⁺ T cells in PP of sham, stroke and stroke + LDC7559 treated old aged mice (n = 3−4 mice per group, ordinary one-way ANOVA, CD19⁺ sham vs stroke **P = 0.0078, stroke vs stroke+LDC7559 *P = 0.0427, CD3⁺ sham vs stroke *P = 0.0183, stroke vs stroke+LDC7559 *P = 0.027). d, Numbers of CD19⁺ B cells and CD3⁺ T cells in spleens of sham, stroke and stroke + LDC7559 treated aged mice (n = 3−4 mice per group, ordinary one-way ANOVA, CD19⁺ sham vs stroke **P = 0.0025, stroke vs stroke+LDC7559 *P = 0.0438, CD3⁺ sham vs stroke *P = 0.0463, stroke vs stroke+LDC7559 *P = 0.0138). Data represent mean ±s.d., All data is combined from at least two independent experiments, PP=Peyer’s patches.