Fig. 7: Inhibition of NETs protects IgA-secreting plasma cells after stroke in young and old mice. | Nature Cardiovascular Research

Fig. 7: Inhibition of NETs protects IgA-secreting plasma cells after stroke in young and old mice.

From: Stroke and myocardial infarction induce neutrophil extracellular trap release disrupting lymphoid organ structure and immunoglobulin secretion

Fig. 7

a, Relative plasma levels of citH3-DNA and NE-DNA complexes in stroke+vehicle and stroke+LDC7559-treated 3-month-old young mice (n = 4−6 per group, citH3 stroke versus stroke+LDC7559 **P = 0.0095, NE stroke versus stroke+LDC7559 **P = 0.0095). b, Numbers of IgD−IgA+CD138− IgA-switched B cells in all PP and SI LP in stroke and stroke+LDC7559-treated young mice (n = 4−6 per group, **P = 0.0095, *P = 0.019). c, Numbers of IgA+IgD−CD138+ plasma cells in all PP and LP in stroke and stroke+LDC7559-treated young mice (n = 4−6 per group, PP comparison **P = 0.0095, SI LP comparison **P = 0.0095). d, Relative concentrations of plasma IgA in stroke and stroke+LDC7559-treated young mice (n = 4−7 per group, **P = 0.0025). e, Relative plasma levels of citH3-DNA complexes in sham, stroke+vehicle and stroke+LDC7559-treated 16-month-old mice (n = 3−4 per group, ordinary one-way ANOVA, ***P = 0.0007, **P = 0.0016). f, Numbers of IgD−IgA+CD138− B cells in all PP and LP in sham, stroke and stroke+LDC7559-treated old mice (n = 3 per group, ordinary one-way ANOVA, PP sham versus stroke *P = 0.0165, stroke versus stroke+LDC7559 *P = 0.0144, SI LP sham versus stroke ***P = 0.0006, stroke versus stroke+LDC7559 ****P < 0.0001). g, Numbers of IgD−IgA+CD138+ plasma cells in all PP and LP in stroke and stroke+LDC7559-treated old mice (n = 3−4 per group, ordinary one-way ANOVA, PP sham versus stroke **P = 0.0014, stroke versus stroke+LDC7559 ***P = 0.0007, SI LP sham versus stroke ****P < 0.0001, stroke versus stroke+LDC7559 ***P = 0.0002). h, Relative concentrations of plasma IgA in stroke and stroke+LDC7559-treated old mice (n = 3−4 per group, ordinary one-way ANOVA, sham versus stroke **P = 0.0019, stroke versus stroke+LDC7559 **P = 0.0061). i, sham or stroke mice were treated with vehicle or LDC7559 every day, and, after 3 d, all mice were intratracheally inoculated with S. pneumoniae (108 CFUs). Mice were euthanized 1 d after infection to analyze bacterial burden in the lungs. j, CFUs in the lungs of infected sham+vehicle, stroke+vehicle and stroke LDC7559 mice (n = 6 per group, ordinary one-way ANOVA, ***P = 0.0003, *P = 0.0195). Data represent mean ± s.d., two-tailed Mann−Whitney U-test (a–d). All data were combined from at least two independent experiments.

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