Extended Data Fig. 9: Experimental model of macrophage-specific PDGFRβ depletion. | Nature Cardiovascular Research

Extended Data Fig. 9: Experimental model of macrophage-specific PDGFRβ depletion.

From: Macrophages upregulate mural cell-like markers and support healing of ischemic injury by adopting functions important for vascular support

Extended Data Fig. 9

a) Schematic representation of the CX3CR1+ cell specific in PDGFRβ KO mouse model (Cx3cr1CreERT2 Rosa26-tdTomato x Pdgfrbflx/flx) where heritable labelling by tdTomato and deletion of PDGFRβ in CX3CR1-expressing macrophages is induced by tamoxifen treatment. b) Gating strategy for macrophages in PDGFRβ macrophage depletion experiments in PDGFRβ KO mouse model (Cx3cr1CreERT2 Rosa26-tdTomato x Pdgfrbflx/flx, related to Fig. 7d). c) Recombinant efficiency of the PDGFRβ KO mouse model (Cx3cr1CreERT2 Rosa26-tdTomato x Pdgfrbflx/flx) showing PDGFRβ expression in tdTomato+ cells. Experimental layout according to the schematic in Fig. 7a.

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