Fig. 2: Single-cell RNA profiling of tdTomato+ and tdTomato-PDGFRβ+ cells in healthy and day 21 post-ischemic muscle. | Nature Cardiovascular Research

Fig. 2: Single-cell RNA profiling of tdTomato+ and tdTomato-PDGFRβ+ cells in healthy and day 21 post-ischemic muscle.

From: Macrophages upregulate mural cell-like markers and support healing of ischemic injury by adopting functions important for vascular support

Fig. 2

a, Experimental layout of the scRNA-seq experiment, where Cx3cr1CreERT2 Rosa26-tdTomato PdgfrβeGFP lineage-traced mice received tamoxifen treatment for 5 days, followed by a 5 day washout period before induction of HLI. Single cells were isolated from healthy mice and at D21 after ischemia induction and thereafter sorted based on tdTomato and PDGFRβ-eGFP signal. UMAP embedding of scRNA-seq data showing the cells colored by tdTomato signal (b), timepoint (healthy or D21 after ischemia) (c) and assigned cell types with macrophage subclustering (d). e, Dot plot of known marker genes for the assigned cell types with the number of cells in each cell type in bar plot to the right. f, Heatmap showing the top five genes driving the separation of the cell type clusters and macrophage subclusters (n = 5).

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