Extended Data Fig. 2: Muscle macrophages downregulate myeloid markers post-ischemia induction.

a) Gating strategy and FMO controls of data from ischemic muscle shown in Fig. 1i and Extended Data Fig. 1J-K. b) Representative image of a PDGFRβ⁺ (green) tdTomato⁺ cell (red) localized between the collagen IV⁺ basement membrane (blue) and CD31⁺ vasculature (white) in the ischemic muscle of Cx3cr1^CreERT2 Rosa26-tdTomato Pdgfrβ^eGFP lineage-traced mice. c) FlowJO UMAP plugin analysis reveals TdTomato⁺ lineage traced cells cluster into PDGFRβ⁺CD11b^lowCD45^lowCX₃CR1^low (black) and PDGFRβ^-CD11b^highCD45^highCX₃CR1^high populations (blue). Cells isolated from day 21 ischemic muscle of Cx3cr1^CreERT2 Rosa26-tdTomato Pdgfrβ^eGFP mice. d) Myeloid markers are reduced with time following ischemia in the tdTomato⁺PDGFRβ⁺ population (left) but not in the tdTomato⁺PDGFRβ^- cells (right) in the Cx3cr1^CreERT2 Rosa26-tdTomato Pdgfrβ^eGFP lineage-traced mouse model. Graphs related to Fig. 1i. n = 4. e) Numbers of cells expressing myeloid markers following ischemia in the tdTomato⁺PDGFRβ⁺ population (upper) but not in the tdTomato⁺PDGFRβ^- cells (down) in the Cx3cr1^CreERT2 Rosa26-tdTomato Pdgfrβ^eGFP lineage-traced mouse model. n = 4. K) Gating strategy and FMO controls of muscle from mice at 3 months post-ischemia induction. One-way ANOVA followed by Turkey test (d-e). Data shown as average ± SEM.