Extended Data Fig. 5: Analysis of in vivo LSEC porosity and targeted lipidomics.
From: Semaphorin-3A regulates liver sinusoidal endothelial cell porosity and promotes hepatic steatosis
a, Representative images of SEM input images (upper panels), calculated probability maps using a machine-learning based approach (WEKA segmentation, middle panels), and outlines (lower panels) used to quantify fenestrae diameter as well as fenestrae frequency, scale bar = 2 µm for the input image (left); 500 nm for the magnification (right). b, Liver ceramide (Cer) and c, Liver diacylglycerol (DAG) profile of chow-fed 35–38-week-old male mice (n = 7 Wt and 6 Sema3a +/- mice). d, Liver Cer and e, Liver DAG profile of diet-induced obese (DIO) control and DIO Sema3a +/- mice kept on a HFD for 20 weeks (n = 7 mice of each genotype). f, Liver Cer and g, Liver DAG profile of DIO iECSema3a and DIO iECwt mice kept on HFD for 20 weeks (with tamoxifen injections after 10 weeks of HFD; n = 4 iECwt and 5 iECSema3a mice). For statistical analysis a two-tailed unequal variances t-test was performed. In all graphs, individual data points and mean ± SEM are presented.
