Extended Data Fig. 6: Lower hepatic fat content in diet-induced obese (DIO) Sema3a +/- mice.
From: Semaphorin-3A regulates liver sinusoidal endothelial cell porosity and promotes hepatic steatosis
a, Experimental plot; b, Body weight; c, Body composition; d, Relative body fat (% of BW) in male DIO wild-type and DIO Sema3a +/- mice, both kept on a HFD for 20 weeks (b–d, n = 10 Wt and n = 12 Sema3a +/- male mice). e, H&E, Picro-Sirus Red (PSR) and Oil Red O (ORO) staining; scale bars = 100 µm. f, Densitometric quantification of hepatic ORO staining (n = 8 Wt and n = 7 Sema3a +/-). g, Liver triglycerides (TG, n = 7 male mice of each genotype). h-m, Serum transaminase and lipid profile, that is AST, ALT, TG, total cholesterol (Chol), HDL, non-esterified fatty acids (NEFA), in n = 6 (h, i, j, k, l) and n = 7 (m) Wt male DIO mice and n = 4 DIO Sema3a +/- mice kept on a HFD for 20 weeks. AST/ALT values displayed as ‘under 15’ were defined as 15 U/L. n, RT-qPCR analysis of genes involved in lipid metabolism in liver tissue from fasted mice. Please note that male, chow-fed 35–38-week-old mice (fasted for 4 h) were used (n = 7 per genotype). o, Intraperitoneal glucose tolerance test (GTT) and area under the curve (AUC), n = 7 mice of each genotype; p, Plasma insulin concentrations during the GTT, n = 7 mice of each genotype. For statistical analysis, a two-tailed, unequal variances t-test was performed in b, d-n, and o (for the AUC blood glucose). A repeated measures two-way ANOVA with Sidak´s post hoc test was used to test for statistical significance in c, o (for the GTT) and p. In all graphs, individual data points, mean ± SEM are presented.
