Extended Data Fig. 7: Metabolic cage analysis of diet-induced obese (DIO) Sema3a +/- mice.
From: Semaphorin-3A regulates liver sinusoidal endothelial cell porosity and promotes hepatic steatosis
a, Absolute and relative food intake in 24 h, normalized to lean mass of DIO control mice and DIO Sema3a +/- mice. b, Absolute and relative water intake in 24 h, normalized to lean mass. c, Physical activity of mice quantified by summarizing photo sensor counts in x, y and z directions separately for day (Light) and night (Dark). d, Oxygen consumption (ml/h/kg body weight) and e, Carbon dioxide (CO2) release (ml/h/kg body weight); f, Energy expenditure (kcal/h/kg body weight). g, Respiratory quotient (VCO2/VO2). N = 10 wt and n = 12 Sema3a +/- mice (20 weeks kept on a high-fat diet) in (a-g). A two-tailed unequal variances t-test was used to assess statistical significance in (a, b); and a repeated measures two-way ANOVA with a Sidak´s post hoc test was used in (c-g). In all graphs, individual data points, mean ± SEM are presented.
