Extended Data Fig. 1: Efficient LSEC isolation from the mouse liver by MACS.
From: Semaphorin-3A regulates liver sinusoidal endothelial cell porosity and promotes hepatic steatosis
a, After isolating CD146-positive liver cells (termed ‘LSEC’) by MACS, cells were allowed to grow for 4 h prior to SEM analysis. Example images are shown for MACS-isolated cells. b, Non-fenestrated CD146-positive liver cells (scale bar = 1 µm). c, Fenestrated CD146-positive liver cells (scale bar = 2 µm). d, e, Enlargement of regions indicated in (b) and (c) (scale bars = 2 µm). For statistical analysis, a two-tailed unequal variance t-test was performed (n = 3 independent LSEC isolations; for each isolation, 60 cells were randomly chosen and analyzed). Individual data points and mean ± SEM are presented.
