Extended Data Fig. 8: APOE Controlled Jag1-induced Elastogenesis and Contraction in VICs.
From: APOE–NOTCH axis governs elastogenesis during human cardiac valve remodeling
a. qPCR analysis of elastogenesis-related genes in VICs with Jag1 treatment; b. Contraction assay of VICs with Jag1 treatment; c. qPCR analysis of contraction-related genes in VICs with Jag1 treatment. Jag1: 15 μg/ml; d. qPCR analysis of FBN2, LTBP1, FBLN2 and EMILIN1 expressions in each NOTCH receptor KD condition; e. Contraction assay of VICs with Jag1 treatment and APOE KD; f. qPCR analysis of contraction-related genes in VICs with Jag1 treatment and APOE KD. Jag1: 15 μg/ml. n = 3 biological repeats in each panel. Data shown as ± SEM. ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001. For a-c, control vs. Jag1 treatment. unpaired 2-tailed t-test (two groups). P value in a: ELN: p = 0.4570, FBN1: p = 0.0902, LTBP1: p = 0.0023, EMILIN1: p = 0.0060, LOXL1: p = 0.0083, FBN2: p = 0.0384, LTBP2: p = 0.7548, LTBP4: 0.0451, FBLN1: p = 0.4267, FBLN2: p = 0.0126, LOX: p = 0.0217; in c: ACTA2: p = 0.0185, MYH11: p = 0.0101, SMTN: p = 0.4497, CNN1: p = 0.0974, TAGLN: p = 0.0096. In d-f: one-way ANOVA followed by Tukey’s test, vs. Scramble. P values in d: FBN2: Scramble vs. siN1: p > 0.9999, vs. siN2: p = 0.0319, vs. siN3: p = 0.9288, vs. siN1 + N2: p = 0.0937, vs. siN1 + N3: p > 0.9999, vs. siN2 + N3: p = 0.0525, vs. siN1 + N2 + N3: p = 0.0029, LTBP1: Scramble vs. siN1: p = 0.7849, vs. siN2: p = 0.0178, vs. siN3: p = 0.2424, vs. siN1 + N2: p = 0.0004, vs. siN1 + N3: p = 0.9567, vs. siN2 + N3: p = 0.0098, vs. siN1 + N2 + N3: p = 0.0035, FBLN2: Scramble vs. siN1: p > 0.9999, vs. siN2: p = 0.0147, vs. siN3: p = 0.7622, vs. siN1 + N2: p = 0.0084, vs. siN1 + N3: p = 0.6843, vs. siN2 + N3: p = 0.0968, vs. siN1 + N2 + N3: p = 0.0022, EMILIN1: Scramble vs. siN1: p = 0.9949, vs. siN2: p = 0.5744, vs. siN3: p = 0.9886, vs. siN1 + N2: p = 0.1343, vs. siN1 + N3: p = 0.9997, vs. siN2 + N3: p = 0.0356, vs. siN1 + N2 + N3: p = 0.0235; in e: Ctrl vs. Jag1: p = 0.3496, Jag1 vs. Jag1+APOE KD: p = 0.0060; in f: ACTA2: Ctrl vs. Jag1: p = 0.0125, Jag1 vs. Jag1+APOE KD: p = 0.0035, CNN1: Ctrl vs. Jag1: p = 0.0002, Jag1 vs. Jag1+APOE KD: p = 0.0006.
