Extended Data Fig. 3: Ultrastructure of HFpEF phenogroups reclassified without accounting for left ventricular hypertrophy.
From: Myocardial ultrastructure of human heart failure with preserved ejection fraction
A) Summary qualitative analysis of electron micrograph assessed fibrosis in the non-failing and the three HFpEF subgroups. Summary qualitative analysis of electron micrograph features for B) sarcomere disruption, lysosome/phagosome abundance, mitochondrial swelling and cristae disruption, C) mitochondrial number, size and area density; and D) Lipid droplet number per field from non-failing (NF) versus HFpEF myocardium. Fibrosis analysis in panel A is based on the primary subgroups presented in Figs. 1 and 2. For panels C-D, HFpEF groups were redefined excluding LV mass information and hence based on those with predominantly elevated systolic blood pressure and the least obesity/DM (Htn-HFpEF), the most prominent obesity/DM but lowest systolic blood pressures (Ob-HFpEF), and a mixed group with equally elevated levels as found in the other two groups (mixed HFpEF). N = 14 non failing, 9 Htn-HFpEF, 9 Ob-HFpEF and 7 mixed-HFpEF. Data shown as mean ± SEM. Statistical analysis: Kruskal-Wallis with Dunn’s multiple comparisons test.
