Extended Data Fig. 4: No damage PF network observed in Sham-operated mice. | Nature Cardiovascular Research

Extended Data Fig. 4: No damage PF network observed in Sham-operated mice.

From: Participation of ventricular trabeculae in neonatal cardiac regeneration leads to ectopic recruitment of Purkinje-like cells

Extended Data Fig. 4

a) Workflow: Cx40-GFP mice are used to study the damages caused by Sham surgery 1 to 3 days, or 21 days after surgery. (b) Open ventricles of Sham-operated mice 2 and 21 days after surgery. The PF network is visualized thanks to Cx40-GFP. (c) No damage of the PF network was observed at the luminal length at any level (successive transverse sections) in Sham-operated mice. (d) Transverse sections at 2 and 21 days after surgery. The PF network is shown in green with Cx40-GFP at 2dpi and Cntn2 at 21 dpi. Although some infiltrated immune cells (CD45) are seen in the myocardium at 2dpi, no accumulation can be observed, and no loss of Cx40-GFP expression in subendocardial myocardium can be seen neither – contrary to MI-operated mice. At 21 post Sham surgery, WGA staining can be observed in-between CMs, although no WGA rich area can be seen, contrary to MI-operated mice.

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