Extended Data Fig. 2: Padi2 and Padi4 expression in mouse brain is minimal.
(a) Representative images of HBMVECs (shScramble, top and shCCM3, bottom) treated with vehicle control (left) or 6 μM Cl-amidine (right) for 24 h. Stained for vWF (white), B-catenin (magenta), and DAPI (blue). Arrows highlight vWF strings. (b) Quantification of the number of vWF strings per field of view in shScramble (shScr) and shCCM3 HBMVECs treated with vehicle or 6 μM Cl-amidine. Each data point represents 1 biological replicate (n = 3) with three fields of view imaged and quantified per condition. The bar indicates the mean of each group, and the error bars represent the standard deviation. Statistical significance was determined using one-way ANOVA followed by a post-hoc Holm-sidak’s test. (c) Quantification of the number of total vWF area per field of view in shCCM3 HBMVECs treated with vehicle or 6 μM Cl-amidine. Each data point represents 1 biological replicate (n = 3) with three fields of view imaged per condition. The bar indicates the mean of each group, and the error bars represent the standard deviation. (d, e) Gene expression of Padi4 in mouse brain cerebellum (D) and vascular and perivascular cells (E). (f, g) Gene expression of Padi2 in mouse brain cerebellum (F) and vascular and perivascular cells (G). (h, i): Bulk RNA-seq data of Padi4 (H) and Padi2 (I) in mouse brain endothelial cells. The error bars represent the standard deviation. (J-K) single cell RNA-seq data of Padi4 (j) and Padi2 (k) in mouse brain cells; Thresholds: blue signifies log2FC ≥ 0.5 & padj ≤ 0.05, red signifies log2FC < 0.5 & padj ≤ 0.05, black signifies p value > 0.05. (l) Correlation analysis of lesion burden to NET burden characterized by citH3+MPO + DAPI+ signals. (m) Logistic regression graph showing the relationship between receiving treatment (1) or not (0) and lesion burden. OR: odds ratio, p: significance level.
