Extended Data Fig. 4: Thrombomodulin and Annexin A5 expression in mouse brain is not endothelial specific.
(a) Quantification of TER-119+ leakage in the cerebellum parenchyma of Ccm3-iECKO vehicle (n = 13) and Cl-amidine (10 mg/kg/day; n = 8) treated mice. (b, c) Correlation analysis of thrombomodulin (THBD) (b) and fibrin (c) expression to lesion burden. (d) Gene expression of thrombomodulin (Thbd) in mouse brain vascular and perivascular cells (e) Quantification of total thrombomodulin (Thbd) expression in the cerebellum (cb) of Ccm3-iECKO vehicle and Cl-amidine (10 mg/kg/day) treated mice. (f) Gene expression of annexin A5 (Anxa5) in mouse brain vascular and perivascular cells. (g) Quantification of total annexin A5 expression in the cerebellum (cb) of Ccm3-iECKO vehicle (n = 13) and Cl-amidine (10 mg/kg/day; n = 8) treated mice. In the graphs (A, E, G), each data point represents one biological replicate, the bar indicates the median of each group, and the error bars represent the interquartile range. Statistical significance was determined using a Mann–Whitney U test (two tailed).
