Extended Data Fig. 7: Kinetic analysis of clots in Ccm3-iECKO mice and characterization of organized clots in sporadic human CCM biopsies.
(a–d) Representative images of Ccm3-iECKO mice aged P11 (No NETs; a), P13 (NETs present; b), P15 ((c), P21 (d), stained with DAPI (blue), fibrin (green) and α-SMA (red). (e) Quantification of organized clot area in the cerebellum (cb) of Ccm3-iECKO P11, P13, P15-28 mice. Each data point represents one biological replicate (n = 9-13 mice per group), the bar indicates the median of each group, and the error bars represent the interquartile range. Statistical significance was determined using a Kruskal-Wallis test followed by a post-hoc Mann-whitney test (f) Representative image of organized clots in sporadic human CCM stained with DAPI (blue), CD31 (magenta), and CD45 (green) showing CD45+ cell infiltration of organized clots. (g) Representative image of organized clots in sporadic human CCM stained with DAPI (blue), CD31 (green), and carbonic anhydrase (CA9, white) showing increased CA9 deposition in organized clots. (h) Representative image showing endothelization of organized clots in sporadic human CCM stained with vWF (green), and the angiogenesis marker, CD93 (magenta). Inserts with white line are shown in the magnifications to the right. For images f-h, the stains were repeated independently in all the samples (n = 4) with similar results.
