Extended Data Fig. 5: The cellular and molecular signatures of lymphoid cells in cardiac inflammation.
a) Box plots showing lymphoid state frequencies in LV per patient in Non-COVID-19 ( = 8), Post-COVID-19 (n = 10), Post-Vaccination (n = 4), MIS-C (n = 2), and control (n = 18) groups. Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values are shown in Fig. 4b. b) Dot plot showing the expression of the top five marker genes for each lymphoid cell state. Dot size corresponds to fraction (%) of expressing nuclei; colour indicates scaled mean expression levels. c) Cytotoxicity (right) or cytotoxic cytokines cores (left) across aggregated CD4+ or CD8 + T cells (control: n = 11; Non-COVID-19: n = 7; Post-COVID-19: n = 9; Post-Vaccination: n = 4). Scores were calculated across ‘cytotoxicity’37 and ‘cytotoxic cytokine’38 gene sets, respectively. Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values were calculated using the two-sided Wilcoxen rank-sum test and were adjusted for multiple testing (Bonferoni correction). d) Dot plots showing expression of MHC-I genes across conditions in CM, EC, FB and mural cells. e) MHC-I gene expression score across aggregated non-immune cardiac cells shown as described in c); control: n = 18, Non-COVID-19: n = 8, Post-COVID-19: n = 10, Post-Vaccination: n = 4. Scores were calculated across all MHC-I genes. P-value calculations as described in c). f) Dot plots as described in b) showing differential expression of RAET1E in CM, EC, FB and murals cells in patient groups relative to control.
