Extended Data Fig. 6: The cellular and molecular signatures in cardiomyocytes and vascular cells in cardiac inflammation.
a) Box plots showing CM state frequencies in LV per patient in Non-COVID-19 ( = 8), Post-COVID-19 (n = 10), Post-Vaccination (n = 4), MIS-C (n = 2), and control (n = 18) groups. Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values are shown in Fig. 5b. b) Dot plot showing the expression of the top five marker genes for each CM state. Dot size corresponds to fraction (%) of expressing nuclei; colour indicates scaled mean expression levels. c) Box plots showing EC state frequencies as described in a). P-values are shown in Fig. 5f. d) Dot plot as described in b) showing the expression of the top five marker genes for each EC and mural cell state. e-g) Dot plots as described in a) showing the expression of e) selected EC10-enriched genes f) MHC-I/-II genes and g) INF response genes across EC states. h, i) UMAP showing expression levels of selected genes per vascular cell nuclei across control and patient groups. j) Dot plots as described in a) showing the expression of MHC-I genes across EC states. k, l) Bar plots showing significant gene sets for EC10 from the k) GO Biological Process 2021 and l) KEGG 2021 Human gene lists. -log10 of the FDR is shown on the x-axis. P-values were calculated using GSEAs Fisher exact test. P-values were adjusted for multiple-testing using the Benjamini-Hochberg correction. m) Circle plot representations of significantly differential interactions of cell-cell communication for the ANGPT1 and ANGPT2 pathways (adjusted P-value ≤ 0.05) in patient groups compared to control. The line thickness represents the interaction strength of signals from sending and receiving cells. Colour is scaled from zero to maximum in disease versus controls (orange, increased; blue, decreased). The directionality of the signal is indicated with arrows. P-values were computed from the one-sided permutation test. n) Stacked bar plots showing the relative contribution of ephrin signalling ligand-receptor (L-R) pairs to the EPHB net signalling related to Fig. 4i in patient and control groups.
