Extended Data Fig. 7: The cellular and molecular signatures in fibroblasts in cardiac inflammation. | Nature Cardiovascular Research

Extended Data Fig. 7: The cellular and molecular signatures in fibroblasts in cardiac inflammation.

From: The cellular and molecular cardiac tissue responses in human inflammatory cardiomyopathies after SARS-CoV-2 infection and COVID-19 vaccination

Extended Data Fig. 7

a) Condition-split UMAP showing transcriptional shifts in FB states across patient groups and controls. b) Box plots showing FB state frequencies in LV per patient in Non-COVID-19 ( = 8), Post-COVID-19 (n = 10), Post-Vaccination (n = 4), MIS-C (n = 2), and control (n = 18) groups. Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values are shown in Fig. 5c. Dots show proportions per patient. c) Dot plot showing the top five marker genes of each FB state. Dot size corresponds to fraction (%) of expressing nuclei; colour indicates scaled mean expression level. d) Dot plots showing differential expression of selected interferon-γ response genes in patient groups relative to controls across FB states. Dot colours indicate log2-transformed fold changes (log2FC). Dot sizes indicate absolute log2FC. Black circles indicate significance (FDR ≤ 0.05). Cell types and genes are ordered alphabetically. e) TGFB Activation score within the vFB2 state across patient and control groups (Control: n = 18, Non-COVID-19: n = 8, Post-COVID-19: n = 10, Post-Vaccination: n = 4). Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values were calculated using a two-sided wilcoxon rank-sum test. Dots show scores per patient. f) Box plot showing snRNA-seq pseudo-bulk expression levels of TGFB2 in cardiac tissue from patient and control groups (Control: n = 18, Non-COVID-19: n = 8, Post-COVID-19: n = 10, Post-Vaccination: n = 4). Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values are indicated for significant changes compared to control, FDR ≤ 0.05. P-values were calculated using the quasi-likelihood F-test and were adjusted for multiple testing (Benjamini Hochberg).

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