Extended Data Fig. 2: Cell-type composition of EMBs and control transmural cardiac tissues.
a) Dot plot shows expression of the top five selected marker genes for each cardiac cell type. Dot size corresponds to fraction (%) of expressing nuclei; colour indicates scaled mean expression level. b) Stacked bar plots showing the cell type composition of each sample. The numbers above each bar show the total number of recovered nuclei. c) Upper left panel: Box plot showing PKHD1L1 + EC frequency in LV for patients and control groups (Control: n = 18, Non-COVID-19: n = 8, Post-COVID-19: n = 10, Post-Vaccination: n = 4, MISC: n = 2). Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. Upper right panel: UMAPs highlighting endocardial EC (EC7) or lymphatic EC (EC8) cell states within the EC_PKHD1L1+ cell population. All other cell types are shown in grey; Lower left panel: Stacked barplot showing EC state distribution (%) within the EC_PKHD1L1+ population (top) and splitted by condition (bottom); Lower right panel: Dot plot shows marker genes of EC7 and EC8 cell states. Dot size corresponds to fraction (%) of expressing nuclei; colour indicates mean expression level. d) Box plots showing cell type frequencies in LV per patient and control groups (Control: n = 18, Non-COVID-19: n = 8, Post-COVID-19: n = 10, Post-Vaccination: n = 4, MISC: n = 2). Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values are shown in Fig. 1d.
