Extended Data Fig. 3: Elevated gene expression of pro-inflammatory markers.
a) Matrix plot showing IFNG expression levels per cell-type. The Colour scale corresponds to the average expression level per cardiac cell-type. b,c) Box plots showing snRNA-seq expression levels (pseudo-bulk) in cardiac tissue from control (n = 18), Non-COVID-19 (n = 8), Post-COVID-19 ( = 10) and Post-Vaccination (n = 4) of (b) differentially expressed cytokines, chemokines and (c) components of the inflammasome complex. Boxes depict the interquartile range (IQR), horizontal bars represent the median, whiskers extend to 1.5 × IQR, dots show the value of each patient. P-values were calculated using the quasi-likelihood F-test and were adjusted for multiple testing (Benjamini Hochberg). d) Pie charts comparing mean absolute expression levels of inflammatory marker genes that are upregulated particularly in the Post-Vaccination group (NLRP1, NLRP3, CASP1, CASP4). The pie size reflects absolute detection levels, colours indicate the relative contribution per cell type.
