Fig. 1: BrdU and EdU incorporation is unsuitable for long-term labeling studies in vivo.
From: Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice
a, Schematic for 20 mg kg−1 BrdU or EdU single injection in P1 mice. b–d, Representative immunofluorescence images (c) and quantification of CMs (b) and non-CMs (d) for BrdU/EdU incorporation (n = 3 mice; 500 to 1,000 total CMs per mouse; two-way analysis of variance (ANOVA) with Šidák’s multiple comparisons: P > 0.05). Arrowhead, BrdU/EdU-positive CM; asterisk, BrdU/EdU-positive non-CM. Scale bars, 20 μm. Data are mean ± s.d. e, Schematic for 20 mg kg−1 BrdU/EdU every other day injection in P2 mice. f–h, Representative immunofluorescence images (h) and quantification of CMs (f) and non-CMs (g) for BrdU/EdU incorporation (n = 4 mice; 400 to 1,000 total CMs per mouse; two-way ANOVA with Tukey’s multiple comparisons). Scale bars, 20 μm. Data are mean ± s.e.m. i, Body weight at the time of injection (n = 4; repeated measures two-way ANOVA with Dunnett’s multiple comparisons). Data are mean ± s.d. j, Immunofluorescence staining of cardiac troponin I (cTnI), γH2AX, and BrdU/EdU with insets. Arrows, γH2AX-positive nuclei. Scale bars, 10 μm. k, Quantification of γH2AX-positive and γH2AX-BrdU/EdU double-positive CMs and non-CMs (n = 2 mice for saline, n = 3 for BrdU/EdU group; 100 to 300 total CMs per mouse; two-way ANOVA with Dunnett’s multiple comparisons). Data are mean ± s.e.m.
