Fig. 2: Genetic tracing of mitoses using CycleTrack.
From: Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice
a, Schematic of the Z/EG reporter for Cre recombinase activation and the AAV vectors carrying either CyB- or CMV-driven Cre recombinase. b, Diagram of the cell cycle of a cell with integrated Z/EG reporter transfected or transduced with CyB–Cre. c–e, Experimental outline (c), representative immunofluorescence images (d), and quantification (e) of C2C12 myoblasts transfected with the Z/EG reporter and either CMV–Cre or CyB–Cre (n = 4 replicates; 1,800 to 3,700 total cells per replicate; Mann–Whitney test, two sided). Scale bars, 100 μm. Data are mean ± s.e.m. f–h, Experimental outline (f), representative immunofluorescence images (g), and quantification (h) of C2C12 myotubes transfected with the Z/EG reporter and transduced with either AAV6–CMV–Cre or AAV6–CyB–Cre (n = 4 different replicates; 650 to 1,100 total myotubes per replicate; Mann–Whitney test, two sided). Scale bars, 100 μm. Data are mean ± s.e.m. i–k, Experimental outline (i), representative immunofluorescence images (j), and quantification (k) of CMs from neonatal Z/EG mice transfected with either cel-miR-67 or hsa-miR-199a-3p and transduced with either AAV6–CMV–Cre or AAV6–CyB–Cre (n = 3 replicates for cel-miR-67, n = 6 for hsa-miR-199a-3p; 990 to 2,200 total CMs per replicate). Scale bars, 20 μm. Data are mean ± s.e.m.
