Fig. 3: CycleTrack to detect mitotic CMs in neonatal and adult mice and after apical resection in neonatal mice.
From: Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice
a,c, Schematic of neonatal (a) and adult (c) Z/EG mice injected, respectively, at P1 or P90 with either AAV9–CMV–Cre or CyB–Cre and 20 mg kg−1 BrdU every other day. i.m.c., intramyocardial; i.p., intraperitoneal. b,d–f, Representative immunofluorescence images for newborn (b) and adult (d) mice and quantification of GFP-positive CMs (e) and their BrdU/GFP double-positive fraction (f) (n = 4; one-way ANOVA with Tukey’s multiple comparisons for e and Mann–Whitney test for f). Scale bars, 100 μm. Data are mean ± s.e.m. g–i, Schematic (g), representative immunofluorescence images (h), and quantification (i) of Z/EG mice injected with CyB–Cre after eventual resection of the heart apex at P1 (n = 4; one-way ANOVA with Tukey’s multiple comparisons). Scale bars, 100 μm. Data are mean ± s.e.m. j, Illustration showing the topographic areas for the assessment of GFP-positive CM regional distribution. k, Quantification of GFP-positive CM distribution in different regions of the heart (n = 3; two-way ANOVA with Šidák’s multiple comparisons). Data are mean ± s.d.
