Fig. 4: CycleTrack detects CM proliferation during pregnancy and after TAC.
From: Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice
a, Schematic of Z/EG female mice receiving either AAV9–CMV–Cre or AAV9–CyB–Cre and eventually mated after 4 days. b,c, Quantification (b) and representative images (c) of GFP-positive CMs in Z/EG female mice receiving AAV9–CMV–Cre (n = 3; Mann–Whitney test, two sided). Scale bars, 100 μm. Data are mean ± s.e.m. d,e, Quantification (d) and representative images (e) of GFP-positive CMs in Z/EG female mice receiving AAV9–CyB–Cre (n = 4; Mann–Whitney test, two sided). Scale bars, 100 μm. Data are mean ± s.e.m. f, Illustration showing the topographic areas for the assessment of GFP-positive CMs regional distribution. g, Quantification of GFP-positive CM distribution in different regions of the ventricles (n = 3 for CMV–Cre and n = 4 for CyB–Cre; two-way ANOVA with Šidák’s multiple comparisons). Data are mean ± s.d. h–k, Schematic (h), quantification (i,k), and representative immunofluorescence images (j) of GFP-positive CMs in Z/EG mice receiving either AAV9–CMV–Cre (i) or AAV9–CyB–Cre (k) after eventual TAC procedure (n = 3 for sham group and n = 4 for TAC group; Mann–Whitney test, two sided). Scale bars, 100 μm. Data are mean ± s.e.m.
