Fig. 5: Visualization of cardiac regeneration upon miRNA treatment after myocardial infarction and mode of CM replication.
From: Genetic tracing and topography of spontaneous and stimulated cardiac regeneration in mice
a–c, Schematic (a), quantification (b), and representative immunofluorescence images (c) of GFP-positive CMs in Z/EG mice receiving AAV9–CyB–Cre and the listed treatments after LAD ligation (n = 4; one-way ANOVA with Dunnett’s multiple comparisons, P < 0.05 day 60 versus day 30). Scale bars, 100 μm. Data are mean ± s.e.m. d,e, Illustration and example image (d) and quantification (e) of GFP-positive CMs found as singlets or doublets at day 60 after infarction (n = 4 for control-treated mice, n = 3 for miRNA-treated mice; two-way ANOVA with Dunnett’s multiple comparisons). Data are mean ± s.e.m. f,g, Illustration and example image (f) and quantification (g) of longitudinal GFP-positive CM doublets likely derived from division along the short or the long axis at day 60 after infarction (n = 4 for control-treated mice, n = 3 for miRNA-treated mice; two-way ANOVA with Dunnett’s multiple comparisons). Data are mean ± s.e.m.
